Friday, September 19, 2014

Dietary Reference Intakes (DRI) & energy, macronutrients, non-nutritive substances, fluid & electrolyte requirements

Nutrition

Science of how living organisms obtain & use food to support all the processes required for their existence.

Nutrient

A substance present in foods used by the body to serve one or more of the following purposes:
  •         Provide a source of energy
  •        Provide structural components
  •        Regulate chemical reactions in the body

Nutrient Density

Is a measure of the nutrients a food provides relative to the energy it provides or /unit weight.
The absence of a nutrient from the diet causes recognizable symptoms – deficiency disease

Non-nutritive substances
  •        Present in foods
  •        Phytochemicals
Plant derived non-nutrient compounds which have biological activity in the body
  •         Zoonutrients
Present in animal foods & provide health benefits beyond provision of essential nutrients & energy
  •         Functional foods
Foods that contain one or more substances thought to have health benefits E.g. Nutrient, phytochemical or zoonutrient
  •       Probiotic foods
Foods & dietary components that contain beneficial live non-pathogenic bacteria
  •       Prebiotic foods
Foods that promote growth of non-pathogenic bacteria

  •       Added to foods
To give flavour, colour, texture &/or freshness eg. Guar gum, colourants

A wholesome diet is needed for the maintenance of the body – Caraka Samhitha (ca 1500 BC)



An adequate (balanced) diet


A diet (combination of foods) which provides energy and all nutrients in correctproportions and in amounts adequate for each age group, gender & physiological state



It should contain both: 



Macronutrients and micronutrients



Macronutrients:

Water

Carbohydrate

Proteins

Lipids

(Minerals)



Micronutrients:
Vitamins
Trace elements



Nutrient recommendations


  •   It is necessary to determine whether amounts of energy & nutrients consumed are likely to be adequate
  •  A set of dietary reference standards developed to assess adequacy of a person’s diet
  • These standards referred to as “Dietary reference intakes - DRIs” encompass 4 sets of values: EARs, RDA, AIs, ULs
  • These dietary standards are used to guide people on amounts to consume


Dietary reference standards: Dietary Reference Intakes (DRIs)

Amounts of energy, nutrients & other dietary components that best support health of nearly all people

Estimated Average Requirement (EAR)
Adequacy of intake
  •         Recommended Dietary Allowances (RDA)
Goal to assure adequacy of intake 

Adequate Intake level (AI)
Adequacy of intake when no RDA is set for nutrient

Tolerable Upper intake Level (UL)
Likelihood of excess or toxicity


Energy requirement of an individual

Adults:
Level of energy intake from food that will balance energy expenditure when the individual has a body size, composition & level of physical activity that is consistent with long term good health

Children & Pregnant or Lactating women:
Requirement also includes energy needs for deposition of tissues or secretion of milk at rates consistent with good health

What are the fuels in foods?

  •          Carbohydrate – 4 kcal/g
  •          Fat – 9 kcal/g
  •         Alcohol – 7 kcal/g
  •         Proteins – 4 kcal/g

Units of energy
Kilocalories (kcal or Cal)
Kilojoules (kj)
Megajoules (MJ)
1 kcal = 4.184 kj
1 MJ = 239 kcal

Energy contribution of a food depends on:

  •          Its energy content
  •          Amount consumed (portion size)
  •          Bioavailability
Energy density = energy content/g of food

Why do we need energy?

Energy requirement depends on energy expenditure
Energy is needed for:
  1. 1       Basal metabolic activities – 50-65% of total energy expenditure
Body’s activities at rest
  1. 2.       Thermic effect of food – 10% of total energy expenditure
Energy required to process food
  1. 3.       Physical activities – 30-50% of total energy expenditure
Occupational & leisure time activities

BMR is affected by:

  •          Growth – children, pregnant & lactating women
  •          Lean body mass – lower in starvation, undernutrition
  •          Gender (lower in females)
  •          Climate
  •          Fever (12% increase in BMR for every 0C rise in body temperature above normal)
  •          Disease (eg. Thyroid hormones increase BMR)

Estimating average energy requirements

Energy – Average requirements recommended, because excess energy:
  •          Cannot be excreted
  •          Is stored as fat

Estimated Energy Requirements (EERs) (defined by IOM,USA) are:

Average energy intakes needed to maintain weight in a healthy person of a particular age, sex, weight, height & physical activity level consistent with good health

Two approaches

A.    WHO/FAO
1. Calculate BMR from equations
2. Multiply by physical activity factor

Eg. Male: 18 – 30 years, weight 60 kg
BMR = 15.3 x 60 + 678 = 1579 kcal/d
  •          Light activity: 1.53 x BMR
Energy req./expenditure = 1579 x 1.53 = 2416 kcal/d
  •          Moderate activity: 1.76 x BMR = 2780 kcal/d
  •          Heavy activity: 2.25 x BMR

B.    Institute of Medicine, Food & Nutrition Board, 2002
1. Use appropriate equation
Insert age in years, weight (wt) in kg and height (ht) in m & physical activity factor
2. For men >= 19 y
EER = [662 – (9.53 x age)] + PAL x [(15.91 x wt) + (539.6 x ht)]
3. For women >= 19 y
   EER = [354 – (6.91 x age)] + PAL x [(9.36 x wt) + 726 x ht)]

Physical activity levels for EER equations

Classify physical activity levels (PAL) depending on lifestyle
Ie. Occupational and discretionary (leisure time) physical activities
Physical activity factors (PAL)



Men
Women
Physical activity
Sedentary
1.0
1.0
Typical daily living activities
Low activity
1.11
1.12
+ 30 – 60 minutes moderate activity
Active
1.25
1.27
+ >= 60 minutes moderate activity
Very active
1.48
1.45
+ >= 60 minutes moderate activity & 60 minutes vigorous activity
Or
120 minutes moderate activity
 
Mod. Activity is equivalent to walking at 3.5 to 4.5 mph

RDA’s for Energy – Sri Lanka (source: MRI, 2007)



Energy (kcal/d)
Infants 0-12 months
Children 1-6 y
Children 6-9 y
               10-11 y (male)
               10-11 y (female)
               12-18 y (male)
               12-18 y (female)
Adults (male): 18-29.9 y
                        30-59.9 y
                        >60 y
Adults (female): 18-29.9 y
                           30-59.9 y
                           > 60 y
Pregnancy
Lactation
560 – 720
875 – 1300
1775
2250
2100
2875-3375
2400-2500
2425-3375 (depending on activity)
2375-3325
1950-2350
2000-2750
1950-2750
1825-2125
+360 - +460 (2nd – 3rd trimester)
+675 (1-6 months), +460 (>6 months)

Energy distribution from nutrients – Population recommendations


Nutrient
% of total energy
Carbohydrates
55-75
   Complex CHO
  50-70
   Refined sugars
    0-10
Proteins
10-15
Fat
15-30
 
Source: WHO, 1990

Nutrient
% of total energy
Total fat
15-30
   Saturates
   <10
   Monounsaturates
10-14
   Polyunsaturates
  3-8
Ratio of Omega 6: Omega 3
<5:1 (3:1)
Trans fats*
          <1
Source: WHO, 1994, ICN 2009


Calculating the energy content of foods, meals & diets

Total energy (kcal) = [carbohydrate (g) x 4] + [protein (g) x 4] + [lipid (g) x 9]

Percent energy from CHO, 
protein or fat                         =        Calories from CHO, protein or fat x 100
                                                                             Total Cal


Carbohydrates

Simple Carbohydrates
Monosaccharides (1 sugar unit)
Glucose
Fructose
Galactose
Disacchharides (2 sugar unit)
Lactose
Sucrose
Maltose
Complex Carbohydrates
Oligosaccharides (3-10 sugar units)
Raffinose
Stachyese
Polysaccharides (>10 sugar units)
Starch
Glycogen
Dietary fiber

  •          Important source of energy
-          65 – 75% of total energy in Sri Lankan diet
  •          Food sources
-          Cereals – Rice, Wheat, Kurakkan
-          Yams – Potato, Manioc, Sweet potato
-          Starchy fruits – Jak, Breadfruit
Provide digestible carbohydrates and fibre (if unrefined)
  •          Digestible carbohydrate
-          Monosaccharides eg. Glucose, Fructose
-          Disaccharides eg. Sucrose, Lactose, Maltose
-          Polysaccharides eg. Starch

  •          Indigestible carbohydrates – Dietary fibre (NSPs)
·         Not digested by human digestive enzymes, May be acted upon by bacterial enzymes (non-carbohydrate lignin also included under fibre)
Recommended dietary intake 27-40 g/d (WHO) (high in soluble fibre)
·         Insoluble fibre
Cellulose, Hemicelluloses, Lignin
·                  Unrefined cereals, Vegetables
·         Soluble fibre
Pectins – Fruits
Gums – Legume seeds


Food
Serving size
Insoluble Fiber (g)
Soluble Fiber (g)
Total Dietary Fiber (g)
Fruits
Guava
Orange
Banana
Mango

100 gm
1 medium
1 medium
1 medium


0.7
1.4


1.3
0.6

4.7
2.0
2.0
3.7
Vegetables
Carrots
Tomato
Potato

1 large
1 small
1 medium

1.6
0.7
0.8

1.3
0.1
1.0

2.9
0.8
1.8
Dark green leafy vegetables 1 cup



approximately 4-5
Cereal Products
All-Bran® cereal
Oat bran
Cornflakes® cereal
Rolled oats
Whole-wheat bread
White bread
Macaroni

½ cup
½ cup
1 cup
¾ cups
1 slice
1 slice
1 cup cooked

7.6
2.2
0.5
1.7
1.1
0.1
0.3

1.4
2.2
0
1.3
0.3
0.3
0.5

9.0
4.4
0.5
3.0
1.4
0.4
0.8
Legumes
Green peas
Kidney beans
Dhal
Lentils

2/3 cup
½ cup
½ cup
1/3 cup

3.3
4.9
4.7
3.9

0.6
1.6
1.2
0.6

3.9
6.5
5.9
4.5

 
See also Box 8 in the FBDG for Sri Lanka
Additional information from www.nal.usda.gov/fnic/foodcomp/data

Fats

  •          Include visible or hidden fats
  •          Concentrated form of energy (9 kcal/g)
  •          Improves taste & texture of food
  •          Supply essential fatty acids
·         Linoleic acid (cis 9,12: C18, omega 6)
·         Alpha-linolenic acid (cis 9,12,15: C18, omega 3)
  •          Provide a vehicle and aid absorption of fat soluble vitamins
  •          Visible fats
·         Butter, Margarine, Cream, Oil
·         Curries, Sauce
·         On meats
  •          Hidden fats
·         Biscuits, Cakes, Chocolates
·         Cheese, Pastry, Some snacks
·         Nuts, Avocado


Fats can divide into three categories

  1. 1.       Phospholipids – FA, Glycerol, P, N base
                 Glycolipids – FA, Sphingosine, CHO
  1. 2.       Triglycerides – FAs + Glycerol
  1. 3.       Derived lipids
                 Sterols – Cholesterol, chol. ester- Chol. + FA
                 Prostaglandins, Thromboxanes, Leukotrienes

Fatty acids can divide into two categories

Saturated fatty acids
·         Palmitic, Stearic
      
 Unsaturated fatty acids
·         Monounsaturated (one cis double bond)
Eg. Oleic, Palmitoleic
·         Polyunsaturated (>one cis double bond)
Eg. Linoleic acid (omega 6), Alfa-linolenic acid (omega 3)
·         Trans fatty acids (trans double bonds)


Unsaturated fatty acids



Fatty Acid composition of some common oils & fats
 
Margarine - SFA - 17%, MUFA - 50%, PUFA - 33%
                                        Pork fat - SFA - 40%, MUFA - 48%, PUFA - 12%


Cholesterol content of some common foods

Chicken giblets - 1 cup - 650 mg
Beef liver - 3 oz. - 325 mg

Egg - 1 - 275 mg
Shrimp - 3 oz. - 150 mg
Salmon - 3 oz. - 125 mg
Crab - 3 oz. - 110 mg
Chicken - 3 oz. - 110 mg
Pork - 3 oz. - 100 mg
Beef - 3 oz. - 90 mg
Lamb - 3 oz. - 80 mg
Oysters - 3 oz. - 70 mg
Ice cream - 1/2 cup - 60 mg
Turkey - 3 oz. - 50 mg

Plant foods do not contain cholesterol


Proteins

·      Thousands of proteins present in the body play many roles
-          Enzymes, Hormones, Receptors, Antibodies, Transporters, Structural proteins etc.
-          Proteins can also provide energy
·      Consist of amino acids linked together by peptide linkages
·      20 AAs commonly found in proteins of which 9 are essential & these must be provided in the food
·      Essential/Indispensable AA
Valine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenyl alanine, Threonine, Tryptophan
Cannot be synthesized in the body, Must be taken in food
·      Non-essential/Dispensable AA
Alanine, Aspartic acid, Asparagine, Glutamic acid, Serine, Arginine, Cysteine, Glutamine, Glycine, Proline, Tyrosine
Normally not essential, May become essential or requirement may increase in some disease states


 Conditionally essential AA

·         Cysteine & Tyrosine in hepatic disease
·         Glycine, Glutamine, Serine & Cysteine in inflammatory states
·         Glutamine – required for GI tract, tissue trauma
·         Arginine – intestinal, metabolic dysfunction & endothelial dysfunction

Protein turnover

·         Continuous synthesis & degradation
·         Rate of turnover relate to the function
E.g. Enzyme proteins – fast
       Structural proteins – long lifetime
Healthy adults who are not gaining or losing weight
I.e. In a steady state with no growth or protein loss are in zero N balance or N equilibrium
N consumed as protein in diet = Amounts lost in urine, faces, other routes
Negative N balance: N Intake < N Output
Positive N balance: N Intake > N Output

Negative N balance in starvation, injury, infection, cancer (loss of body protein)

Chronic illness & trauma
Most protein loss is from skeletal muscle
With preservation of viscera & other proteins
No adaptation
Starvation
Loss of protein affects most tissues similarly
Priority to restrict loss of protein by minimising need for glucose
Brain adapts to use ketone bodies, with decreased protein synthesis in most tissues

Quality of protein

·    Essential AA content compared with required pattern/g protein (limiting AA – lowest amount)
·         High quality: animal proteins eg. Eggs, milk, fish, meat (exception collagen & gelatin)
·         Moderate or low quality: most plant proteins
·         Infants & children need more essential AA /kg body weight than adults
I.e.             Essential AA ratio is higher
                     Total AA


Supplementary action of proteins

If a protein deficient in an essential amino acid is mixed with another protein having the same AA the quality of the diet increases – supplementary action
E.g. Rice is deficient in Lysine
Pulses contain Lysine but moderate or low quality
Lysine deficit in rice overcome by taking pulses/fish, milk products or eggs in same diet
E.g. Soya, Dhal, Mung etc. + Rice,
       Fish/Egg/Curd + Rice,
       Macaroni & Cheese

RNI for protein

Recommended intakes of high quality reference proteins for normal humans (MRI, 2007)

Age (yrs)
RDA (g/d)
0 – 0.5
21
7 – 10
19

Male                          Female
10 – 11
48                               50
12 – 15
69                               65
19+
57                               57
Pregnancy
+ 10 g/d
Lactation (1-6 months)
+ 20 g/d + 14 g/d (> 6 months)


Protein requirement of adults

·         Estimated to be:
0.75 g of high quality, digestible protein/kg body weight for both sexes
·         Requirement of mixed protein:
Approximately 1 g/kg body weight
·         Regular physical activity increases protein requirement to:
1.2 – 1.6 g/kg body weight
Increased requirements usually covered by increased food intake

Fluid & Electrolytes

Water constitutes approximately 60% of adult body weight & higher % of a child’s weight
-          Carries nutrients & waste products throughout the body
-          Participates in metabolic reactions & serves as a solvent
-          Aids in maintaining blood pressure & temperature
-          Maintains blood volume
-          Acts as a lubricant
To support these vital functions, body’s water balance is actively regulated
To maintain water balance intake from liquids, foods & metabolism must balance losses from kidneys, skin, lungs & faeces

Water balance


Water sources
Amount (ml)
Water losses
Amount (ml)
Liquids
550 – 1500
Kidneys (urine)
500 – 1400
Foods
700 – 1000
Skin (sweat)
450 – 900
Metabolic water
200 – 300
Lungs (breath)
350


GI tract (faeces)
150
Total
1450 - 2800
Total
1450 - 2800


Water needs depend on:
-          Foods a person eats
-          Environmental temperature & humidity
-          Activities levels & many other factors
AI for total water (drinking water & beverages & foods):
-          Men 3.7 l/d
-          Women 2.7 l/d
DRI committee


Electrolytes

·         Major minerals from salts that dissolve in body fluids (electrolytes) & cells direct where these salts go
·         Are vital to life of cells & must be closely regulated to maintain appropriate distribution of body fluids
·         Help to hold water in compartments where it is needed
·         Help to maintain appropriate distribution of body fluids & acid base balance

Major minerals

  •          Calcium and Phosphorus are the most prevalent minerals in the body
  •          Although all major minerals influence fluid balance, Na, K & Cl are most noted for this role
  •          Na – principal electrolyte in extra cellular fluid and primary regulator of extra cellular fluid volume
  •          Cl – the major negative ion in extra cellular fluid, salt is a major food source
  •          K – major cation in cells & plays a major role in maintaining fluid & electrolyte balance & cell integrity
Na recommendations and food sources 

  •          Diets rarely lack Na
  •          When intake are low, body adapts by reducing losses in urine & sweat – deficiency unlikely
  •          Na intake should be < 2000 mg/d
                1 tsp (5g) salt contains 2000 mg Na
  •          Processing increases Na content
K recommendations and food sources

Abundant in all living cells
Fresh foods (intact cells), specially fruits & vegetables contain adequate K
Processing leads to decreased K
K intake should be at least 90 mmol/d (3.51 g/d)


Food dietary guidelines Sri Lanka - 2011

Serving Sizes

1 cup = 200ml tea cup

Cereals and Starchy foods
Cooked rice                                    ½ cup – 60g
Bread                                             1 slice (50 g)

Vegetables
Cooked vegetables
(fruit vegetable or leafy vegetable)                   3 tbsp (1/2 cup)
Raw salads                                                          1 cup (200 ml)

Fruits
Medium size fruits                                              1 (1 banana/1 orange)
Cut fruit/fruit salad                                             ½ cup
Dried fruits                                                          2 tbsp (20-30 g)

Fish, pulses, dried fish, egg, poultry and meat
Cooked fish/poultry/meat                                   30 g
Cooked pulses                                                      3 tbsp
Eggs                                                                      1
Dried fish                                                              15 g

Milk & dairy products
Milk                                                                       1 cup (200 ml)
Yogurt/curd                                                           1 cup (100 ml)
Milk powder                                                          30 g (2 tbsp)

Nuts & oil seeds                                                    1 tbsp (15 g)

Cooked items/quantities measured without gravy
Approximately 8 glasses of water


The diet of a Sri Lankan Business Man

Mr. Perera is a busy man who owns his own prosperous business as a building contractor. His typical daily meals are as follows.
Breakfast
4 slices white bread with pol sambol, lunu theldala
Tea with 3 teaspoons sugar and full fat milk
Lunch
4 cups white rice with chicken curry, parippu, papadams, & 1 egg cooked in coconut milk
Afternoon
Tea with full fat milk and 3 teaspoons sugar
Dinner
4 white flour rotis and chicken curry, ala thel dala
Evaluate his diet using the Food Based Dietary Guidelines for Sri Lankans and the following chart
What suggestions would you have for Mr. Pereira about improving his diet?


Evaluation of a diet


Food Group
# of servings consumed
# servings recommended
Within range?
Recommendations for change
Rice, bread, cereals




Fruits




Vegetables




Milk products




Fish, pulses, meats, eggs




Nuts, oils, sugar







References:

  • 1.       Essentials of nutrition & diet therapy Williams & Schlenker (Latest edition)
  • 2.       Understanding nutrition E Whitney, S R Rolfes 11th Edition, USA
  • 3.       Nutritional Sciences from Fundamentals to food, M Mcguire & K A Beerman, Thomson Wadsworth, USA, 2005
  • 4.       Food Based dietary guidelines for Sri Lankans, Ministry of Health, 2011















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